ABSTRACT
Bendamustine hydrochloride is used to treat chronic lymphocytic leukemia. It kills the existing cancer cells and limits the growth of new cancer cells. Three simple, rapid and sensitive spectrophotometric methods were developed for the determination of Bendamustine hydrochloride in phosphate buffer (pH 8.0) (Method A) and boric buffer (pH 9.0) (Method B). Method C is a difference spectroscopy technique in which the amplitude was chosen for the analytical calculations. Bendamustine hydrochloride obeys Beer-Lambert’s law over the concentration range 1-40 μg/ml, 0.1-40 μg/ml and 5- 40 μg/ml with regression equations y = 0.003x + 0.001(r² = 0.998) y = 0.0027x + 0.0005 (r² = 0.999), and y = 0.0034x + 0.006 (r² = 0.994) for Method A, B and C respectively. The methods were validated as per ICH guidelines and can be applied for the determination of Bendamustine hydrochloride in pharmaceutical formulations.
ABSTRACT
Two simple, precise and accurate first derivative spectrophotometric methods were developed for the determination of Zolpidem Tartrate in pharmaceutical formulations in phosphate buffer pH 6.8 and acetate buffer pH 4.0. Beer’s law was obeyed over the concentration range 0.5-20 μg/ml in both phosphate buffer (pH 6.8) (Method A) and acetate buffer (pH 4.0) (Method B). The regression equations were found to be y = 0.101x+0.012 (r2 = 0.999) and y = 0.064x+0.009 (r2 = 0.999) in Method A and B respectively. The % RSD in precision studies was found to be 0.28-0.69 (Intra-day) and 0.31-0.73 (Inter-day) for Method A and 0.25-0.82 (Intra-day) and 0.26-0.57 (Inter-day) for Method B respectively. The % RSD in accuracy studies was also found to be 0.14-0.19 (Method A) and 0.18-0.23 (Method B) with percentage recovery 98.67-99.78 and 98.56-99.83 Method A and B respectively.